FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15618185
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC462290.2959947010488094No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT352810.2258969272037692No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC247960.1587636463519929No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT189160.12111522561680502No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT180050.11528228151990773No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT178900.11454596036607327No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA174800.1119208153828374No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG173420.11103722999823602No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA160570.10280964145321624No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC159090.1018620281421945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT86950.039.9797631
ACTCTAG106350.034.4219675
GACTCTA111200.032.3312234
CTGACTC108450.031.9427052
CTAGCAG115850.030.2847718
TGACTCT140550.025.6043663
CTCTAGC141700.025.3233596
TAGCAGA142800.024.3518379
TCTATCG156050.022.08766416
CTATCGA156050.022.08738117
GATCTAT170600.020.44629714
TATCGAT174150.019.81087918
AGATCTA176700.019.74013913
ATCTATC179200.019.36872715
GCAGATC181000.019.30873911
TCTAGCA289650.018.770597
AGCAGAT194000.018.19199210
GTGCGCA148650.018.14933468
GGTGCGC150150.017.94487467
ATTCTAG197600.017.44176723
Produced by FastQC (version 0.11.2)