FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6424039
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT112810.17560603227969193No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA80640.1255285031737821No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC64330.1001394916811682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG30150.018.6595571
CAAGGGC29950.018.202292
CCCGTCC5550.015.54039733
GGATAAC63350.014.65570451
GCGAGGA41000.014.1357947
GGCCGTT28000.012.56734963
CGTGGCC28650.012.40263960
TGGCCGC43950.012.32440620
GGGCGCC43450.012.306560566
AGGGCGC43900.012.25918665
AGGGGCG35750.012.06282635
AGCGTCG46700.012.04117610
GCGTCGT45950.012.01304111
TAGGGGC35400.011.88972134
GGCGGTC37850.011.66808815
CAGCGTC49400.011.6623949
TCAGCGT49600.011.6153688
CGTTCAC30450.011.55600466
CGTAGGG37350.011.54598732
GGGTGCC37950.011.54570823
Produced by FastQC (version 0.11.2)