FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7781091
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT220300.28312225110848854No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA151840.19513973040541488No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC100050.12858094064187145No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG98180.12617767868284793No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC93330.11994461959126297No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA90210.11593489910348047No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG86640.11134685354534472No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG85750.1102030550728683No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG81640.10492101943030867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG52850.022.9256441
CAAGGGC52400.022.651052
GCGAGGA74500.016.4388687
CGTGGCC46100.014.44305160
GGATAAC116350.014.3891371
GGCCGTT47100.014.2095663
CGAGGAG88250.014.1903058
CGTTCAC46500.014.17024766
CCGTTCA46850.014.06447665
GCCGTTC48100.013.84242164
TGGCCGT51250.013.1263362
GTTCACG49850.013.08033267
GGGGCCC2406.375027E-512.93775669
GCCGGGT6950.012.9136181
TCGTGGC54150.012.74189859
AAGGGCG98350.012.41873
CGGCGCG64650.012.22026733
GCGCGTT63700.012.18580135
TCGGCGC65750.012.06829432
GGCGCGT66800.011.72365934
Produced by FastQC (version 0.11.2)