FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8228319
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT172140.20920433444546813No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA107810.13102311662928967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG44000.017.65181
CAAGGGC46550.016.3063322
GTGTGCG10400.014.9273358
AGGGGGG3605.802576E-1014.376134
CGTGGCC29200.014.1775660
CGTTCAC31100.013.6441166
CGCGTAT10250.013.470861
GTTCACG33200.013.4047967
GCCAGTA16800.013.3555951
GCGAGGA58000.013.3236477
CCGTTCA32450.012.86393465
TCGTGGC33850.012.535727559
AGCGTCG56750.012.5230410
GGGCGCC53500.012.50974466
GGATAAC101750.012.3827651
TGGCCGC56650.012.362920
GCGTCGT58700.012.16609411
CAGGGCG55300.012.04024464
GCGCCGT56250.012.021111568
AGGGCGC56250.011.9595665
Produced by FastQC (version 0.11.2)