FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10234768
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT294830.28806710616205466No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA205550.20083503602621963No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC136210.13308557653676176No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG123390.12055964531878006No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC122600.11978776656197776No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA121060.118283091517072No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG117720.11501970538071796No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG114480.11185402541611104No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG108260.10577670153343974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG71900.021.8424661
CAAGGGC74950.020.8526422
GCGAGGA100500.015.8590267
CGTGGCC52550.014.8368160
GGATAAC156200.014.517891
GGCCGTT53550.014.30205163
CGCGTAT16900.014.2965251
CGTTCAC53300.014.11016366
CCGTTCA54650.013.95105265
GCCGTTC56450.013.93399864
CGAGGAG120000.013.598378
GTTCACG55750.013.55221867
CGTATTA21250.013.1509923
CCGCGCG14450.012.8923162
TGGCCGT62050.012.62085862
TCGTGGC63100.012.57486359
ACTCGTG75800.012.51638668
CGCGCGA14650.012.48081965
AGGGGCG78750.012.17876735
GCGCGAT16350.011.81606866
Produced by FastQC (version 0.11.2)