FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10243396
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT216450.21130687518084823No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA141600.138235405523715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG57300.021.3268281
CAAGGGC59100.020.491642
GCGAGGA81350.015.1819467
CGTGGCC43950.014.67881760
GGCCGTT44850.014.53810163
GGATAAC120550.014.3465721
CGTTCAC43900.014.22388166
GCCGTTC47150.013.68258764
CGAGGAG96950.013.201718
CCGTTCA47900.013.18025765
TGGCCGT53100.012.79911662
GACGTAC22050.012.36047956
AAGGGCG100850.012.31621553
GTTCACG50200.012.30172767
TCGTGGC54350.012.25084559
GTCGAAT23500.012.0454661
CGCGTAT12950.011.9955561
GATAACA153200.011.3058192
ACTCGTG60400.010.966457
ATAACAT155000.010.9517653
Produced by FastQC (version 0.11.2)