FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8463637
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC274190.3239623816569638No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT188170.22232758800974098No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC132820.15693017080009458No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT102690.12133081794505128No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT94040.11111062537299272No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT89940.10626637224635226No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA87680.1035961254009358No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG86250.10190654443237582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT50300.040.9027331
ACTCTAG63200.034.174155
CTGACTC61900.033.443272
GACTCTA66700.031.7084564
CTAGCAG66650.031.1084868
TAGCAGA78800.026.268319
TGACTCT81150.025.8498543
CTCTAGC82650.025.7145166
CTATCGA89250.022.57630217
TCTATCG89950.022.24731416
GATCTAT99350.020.3853614
AGATCTA101550.020.0454213
ATCTATC102350.019.88885515
TATCGAT101900.019.80692318
GCAGATC106350.019.10756911
AGCAGAT109300.018.93851510
TCTAGCA168900.018.4849557
TAGCATG111000.018.24441527
ATTCTAG111700.018.16107623
CTAGCAT114700.017.71614826
Produced by FastQC (version 0.11.2)