FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7152406
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT153680.21486476019398226No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA81950.11457682911177021No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG71760.10032987500989178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG35900.015.8660831
CAAGGGC37000.015.2003472
GGCCGTT26800.015.1896663
CGTGGCC27400.014.98294760
GGATAAC74150.014.8045841
CCGTTCA29800.013.89203465
CGTTCAC30300.013.77655266
GCCGTTC31000.013.57684864
GTTCACG33750.012.47154667
AGGGGCG38300.012.43054735
TGGCCGT34150.012.32451862
TAGGGGC39750.012.23747734
TCGTGGC34350.012.15241259
CGTAGGG40850.011.90794932
GGGCGCC45050.011.86956266
GCGAGGA48850.011.8642287
AGGGCGC44900.011.755630565
ACTCGTG41550.011.62503668
GATAACA98550.011.6238262
TAACATG91350.011.5576994
Produced by FastQC (version 0.11.2)