FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47994633
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1488230.3100825877760124No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1201480.2503363240635677No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG803960.1675103964228667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGAA1067350.057.4579247
GAATCTA513950.052.53650711
GTTTCGT278650.050.1868487
TTGAATC1220400.050.026339
CAATCTA414450.048.60655211
TGAATCT1277400.047.90703610
CTTGAAT1335200.046.0786448
CACTTGA299650.042.352736
GTCAATC612550.042.2842569
CCATCTA210600.038.4880911
AATCTAT447700.037.8125212
AGGGGGG183550.037.5576361
TACTTGA505950.037.507646
GAATCTT363450.037.00597811
AATCTAA498550.035.87928412
TGTTTCG153600.035.569656
AATCTAG241200.035.56416712
CGTTTCG77100.035.3177536
GAATCTG251450.034.935611
CGACTTG73350.034.546685
Produced by FastQC (version 0.11.5)