FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-vec..ph..bZIP63_Col..h_B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-vec..ph..bZIP63_Col..h_B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581202
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT11680.20096283219947628TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGTT7690.13231200167927845TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCAG18600.060.0264433
CCAGTCA18600.060.0264426
TCCAGTC18450.059.7556625
CAGTCAC18950.058.9177727
ACTCCAG18950.058.73307423
CGGGGTG4000.058.6245653
CTCCAGT18850.058.48763324
CACATTC19400.057.9119531
GAACTCC19450.057.58312221
ACGTCTG19500.057.25598515
AGTCACA19600.057.14243328
CCGGGGT1350.057.03661752
CGTCTGA19650.056.8189216
GTCACAT19450.056.68338829
CACGTCT19800.056.5652414
GCACACG20000.056.5245811
CTGAACT19700.056.49704419
CACACGT19950.056.31537212
ACACGTC20000.056.1745813
TCTGAAC20050.055.51081518
Produced by FastQC (version 0.11.5)