FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-vec..ph..bZIP63_Col..h_B_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-vec..ph..bZIP63_Col..h_B_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423974
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT10090.23798629161222148TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGTT7000.16510446395297823TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTTT4680.1103841273285626TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT4540.10708203804950303TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGCGGTT4420.10425167581030913TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGG1150.066.9558451
GCGGGGT2700.064.81415652
CATTCAG15000.061.36604733
CGTCTGA15600.058.78145616
CGGGGTG3050.058.52399453
CAGTCAC15350.058.1427427
ACGTCTG15850.058.07512715
CACGTCT15850.058.07512714
GCACACG16000.057.96816311
TCCAGTC15550.057.61999525
CACACGT16100.057.39072412
GTCTGAA16050.057.1333817
CTCCAGT15900.056.5717624
ACACGTC16350.056.29912613
GTCACAT16150.056.1294629
TATGCCG1500.055.99943548
ACTCCAG16000.055.9994323
CCAGTCA15950.055.9555526
CTGAACT16400.055.9140719
AGCACAC16600.055.8729310
Produced by FastQC (version 0.11.5)