FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-vec..ph..bZIP25C_Col..h_B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-vec..ph..bZIP25C_Col..h_B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614725
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT15550.25295864004229535TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGTT9490.15437797389076416TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTTT7620.12395786733905405TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT6850.1114319411118793TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTTT6450.10492496644841189TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGTCGTAT6230.10134613038350483TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCAC24150.060.57668327
CCAGTCA24300.060.05872726
TCCAGTC24450.059.54712725
CTCCAGT24700.059.0861224
GTCACAT24950.058.9148929
ACTCCAG24900.058.9022223
CATTCAG25050.058.40027633
GCGGGGT4700.058.08220352
AGTCACA25350.057.98526828
TATGCCG2000.057.74711248
ACGTCTG25600.057.70181315
ACACGTC25950.057.19334813
GCACACG25950.057.19334811
CGTCTGA25900.057.0334616
CACACGT26000.056.94872312
CACGTCT26200.056.64760614
CACATTC26150.056.34517731
CTGAACT26450.055.97984319
GAACTCC26450.055.84750421
CGGGGTG5300.055.4689353
Produced by FastQC (version 0.11.5)