FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP9..ph-vec_Col..h_B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP9..ph-vec_Col..h_B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423603
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT16320.3852663932974979TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC5450.12865820119309826TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3300.043.4890350
CCGTCGT1150.042.61279352
ATGCCGT3450.042.61279349
GCCGTCT2150.042.32964751
CGTATGC3550.041.41243446
TATGCCG3600.040.8372648
GCCGTCG1200.040.83725751
GTATGCC3750.039.20376647
TCGTATG4050.035.4313245
CGGCAGA400.002417974934.99923318
GATCTCG3850.034.54469741
CCGTCTT2850.034.3892752
CTCGTAT3800.034.078244
TTACTCG5650.033.45059235
ACTCGAT4950.032.5245437
CAGTCAC5700.031.92912727
GGCCACC450.00430588331.11042831
ATTACTC6000.030.33266834
GTCACAT6150.030.1619429
CGATCTC4200.029.99934440
Produced by FastQC (version 0.11.5)