FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP9..ph-bZIP63_Col..h-B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP9..ph-bZIP63_Col..h-B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464964
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1301.6552804E-1029.61447345
GCAGGGC500.00720710227.9991414
GTATGCC1554.3655746E-1127.09594344
TGCCGTC1305.3951226E-926.92224950
GTTACGT750.001831724423.33261770
GCCGTCT1253.0862884E-622.39931151
CGTATGC1957.767085E-1021.537843
ATGCCGT1656.780101E-821.2114746
TCGTATG2051.4497346E-920.48717542
CTCGTAT1801.6979538E-719.44384841
GATCTCG1651.5894584E-619.09032241
CAGTCAC2801.0913936E-1118.74942424
AGTTACG950.007205583618.42048869
TTACTCG2651.873559E-917.16928332
CCGTCTT1653.389568E-516.96917552
GTGTGGA1300.00228851216.15334963
TACAGGA1300.00228851216.15334933
TAGCCTT1605.155114E-415.3120353
CGATCTC2151.9710063E-514.65071237
GAAGGAC1450.00473524114.48231340