FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP9..ph-bZIP25_Col..h-B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP9..ph-bZIP25_Col..h-B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107785
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6350.027.0071645
GTATGCC6500.025.30702244
ATGCCGT6350.024.25132846
TGCCGTC5850.023.9309947
CGTATGC6950.023.6684443
GCCGTCT3700.023.64802248
CTCGTAT6650.020.52577441
TCGTATG8250.019.09040542
TTACTCG10500.017.99995232
CCAGTCA12250.016.00071523
CAGTCAC12150.014.97973824
GATCTCG8650.014.97070238
TCTCGTA8850.014.63238240
TACTCGA13700.014.3065333
TCCAGTC14100.014.14923422
CGATCTC10100.013.8610237
GTCACAT14850.013.19893126
CCGTCTT7500.013.06632149
ACTCCAG15400.012.95481820
ACTCGAT14450.012.59482234
Produced by FastQC (version 0.11.5)