FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP9..ph-bZIP10_Col..h-B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP9..ph-bZIP10_Col..h-B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218290
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4750.024.31563245
ATGCCGT5100.021.2743746
CGTATGC5600.020.62486643
GTATGCC5850.020.3417544
TGCCGTC4700.020.10625347
GCCGTCT4550.019.99987248
CTCGTAT7450.016.44284841
TCGTATG7400.016.08097842
CCGTCTT6800.013.38226749
ATCTCGT10050.012.88548939
GATCTCG9600.012.76033438
TTACTCG11850.012.404983532
CGATCTC9950.011.95972237
CAGTCAC12100.011.85942824
TCTCGTA11250.011.51103640
CCAGTCA14350.010.48773723
CTCCAGT14250.010.31572221
ACTCCAG14800.010.16885420
TCCAGTC14250.010.0701122
ACATTAC15500.09.9354229
Produced by FastQC (version 0.11.5)