FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP63..ph-vec_Col..h_B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP63..ph-vec_Col..h_B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308862
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT11890.3849615685969786TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC3740.12108967759063918TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2300.039.56265646
TATGCCG2450.035.71197548
GTATGCC2550.035.68396447
CCGTGGT400.002417471534.99773452
ATGCCGT2400.034.99773449
TCGTATG2700.034.9977345
CTCGTAT2850.034.38373644
TTACTCG3650.032.60062835
GATCTCG2700.032.40530841
CTGCAGG450.00430499631.10909855
CCGCATA450.00430499631.10909830
CGATCTC2750.030.54347640
CTCGATC3100.030.48189738
ATCTCGT2900.030.1704642
TCTCGTA2800.028.74813843
TGCACTA500.007204612327.99818831
CATTACT4250.027.99818633
TGCCGTC2400.027.7065450
CACATTA4350.027.3545531
TCGATCT3350.027.1624239
Produced by FastQC (version 0.11.5)