FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP63..ph-bZIP9_Col..h-B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP63..ph-bZIP9_Col..h-B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699376
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4000.023.62479245
TGCCGTC3950.023.03777147
GCCGTCT2650.022.45263348
ATGCCGT4100.022.19492746
GTATGCC4400.019.0907444
CGTATGC4500.018.66650243
CTCGTAT5400.015.55541841
TCGTATG6150.014.22751642
TTACTCG8150.012.45387832
ACGGAGG2254.7512582E-412.44433521
TCTCGTA6850.012.26266640
CAGTCAC8500.011.94107124
TCCAGTC9950.011.25618322
GGCGTGA2200.005671286511.13626557
CCAGTCA9600.010.57282423
GATCTCG7301.8189894E-1210.547852538
TACTCGA10050.010.44766933
CCGTCTT5401.7067578E-810.37027949
CTCCAGT10950.010.2282221
CGATCTC7901.8189894E-1210.18978337
Produced by FastQC (version 0.11.5)