FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP53..ph-bZIP9_Col..h-B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP53..ph-bZIP9_Col..h-B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134904
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGG4150.024.45741729
ATCGGGG3200.021.87462839
CGGGGGG3400.021.61728141
CACTCCG4650.021.0749128
TCGGGGG3350.020.89516840
TCCGGAG5150.019.02880131
GTCACTC5850.018.54669226
GAATCGG4600.017.49970237
CTCCGGA5700.017.1926930
CCGGAGA6450.016.27879332
AATCGGG4650.015.80618238
CAGTCAC6650.015.78920624
CCGTCCG1350.002946908115.55529128
CGGAGAA6650.015.26289833
TCCAGTC6850.014.8179222
TCACTCC7050.014.39691927
CCAGTCA7100.014.29616223
AGTCACT7850.013.37556925
CTCCAGT7500.013.0670221
ACTCCAG7700.012.72761720