FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP53..ph-bZIP25_Col..h-B_q1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP53..ph-bZIP25_Col..h-B_q1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831252
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG2600.034.99942839
CGGGGGG2700.031.11060141
TCGGGGG2800.028.7495340
CACTCCG4000.027.12455728
ACTCCGG4150.026.9875129
AATCGGG3800.023.94697638
TCCGGAG4750.023.57856231
CCGGAGA5100.022.64668832
CTCCGGA5100.021.96042630
TCCAGTC5150.021.74721722
GTCACTC5000.021.69964426
CAGTCAC5350.020.93423724
TCACTCC5400.020.740427
GAATCGG4600.020.54314237
CCAGTCA5600.019.99967423
CGGAGAA5900.019.57595333
AGTCACT6100.017.78659425
AGAATCG5550.017.6573736
TGCTGGG1000.00968909517.49971432
CTCCAGT6550.017.09895721
Produced by FastQC (version 0.11.5)