FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP44..ph..bZIP25C_Col..h_B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP44..ph..bZIP25C_Col..h_B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192297
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT122261.0254156472758047TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT59030.4950947624627085TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTTT50120.4203650600479578TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTTT38750.3250029145422659TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGGAT34250.2872606405954221TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGTT31140.26117653571215893TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTAT29180.24473767861531145TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGTTT27760.2328278943920852TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGGTT26950.2260342850816533TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGGAT26820.22494395272318896TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTTT26190.2196600343706308TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGTAT22730.19064041929150202TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGAT21090.1768854572308745TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGGTT17090.1433367692781245TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGTT13730.11515587139781447TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGAT13500.11322682184053134TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGGT12400.10400093265352509TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG37950.066.309939
TATCGCG22100.065.88132541
AGTCACG89100.065.5208628
GTCACGA89100.065.4815829
GTATCGC23050.065.44366540
CAGTCAC90550.064.7422327
TCGTATC78850.064.67242438
CCAGTCA90650.064.63219526
TCCAGTC91100.064.3513625
CACGAAT89800.064.3475531
ACTCCAG91350.064.2518823
CTCCAGT91900.063.94351224
ATTCGTA89400.063.61757736
TCACGAA91800.063.59377730
CGAATTC90800.063.21487833
ACGTCTG92750.063.05826615
GAATTCG90800.063.0221534
TTCGTAT86450.062.9949737
GAACTCC93100.062.7433921
GCACACG93650.062.63912611
Produced by FastQC (version 0.11.5)