FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP44..ph..bZIP25C_Col..h_B_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP44..ph..bZIP25C_Col..h_B_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756703
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT114741.5163148553659758TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT56750.7499639885133269TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTTT48100.6356522968720886TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTTT36440.4815627795845927TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGGAT33540.4432386286297266TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGTT30640.40491447767486055TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGGTT28150.37200856875154453TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTAT27440.36262575937983593TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGGAT25900.3422743137003554TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGTTT25300.33434517902003824TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTTT23650.31254005864916623TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGTAT21370.2824093468639612TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGAT20210.26707968648201474TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGGTT15980.21117928698577906TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGAT13240.1749695719456643TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGGT12670.16743689399936304TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGTT12200.16122573849978128TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG15150.067.2272840
ATCGGGG7700.065.9086542
AGTCACG80700.065.6625128
GTCACGA80700.065.6625129
CGTATCG35900.065.51488539
TCGTATC69700.065.3295538
CCAGTCA81500.065.1468126
ATTCGTA79250.065.1415336
CGAATTC80850.065.10779633
CACGAAT81500.065.01797531
ACTCCAG81700.064.98733523
TTCGTAT76750.064.9376837
GAATTCG81100.064.77762634
TCCAGTC82100.064.6707125
CGTATCT34300.064.5914139
CAGTCAC82550.064.5301627
ACGAATT82050.064.4541832
AATTCGT80850.064.32857535
CTCCAGT82600.064.27923624
TCACGAA82850.064.00078630
Produced by FastQC (version 0.11.5)