FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP25..ph-vec_Col..h_B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP25..ph-vec_Col..h_B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240619
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG7900.32831987498909065TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGA150.002222761370.035
TCGGGGG1450.045.8620743
ATCGGGG1600.043.7542
ACTCCGG1950.043.07692332
CTCCGGA2100.041.66666833
CGGGGGG1350.041.4814844
TCCGGAG2100.040.00000434
CACTCCG2150.039.06976331
TCCAGTC2250.038.8888925
GTCACTC2250.038.8888929
AATCGGG1850.037.83783741
AGTCACT2400.036.45833228
GAATCGG1950.035.89743440
TCACTCC2350.035.74468230
CGGGGGT400.002415645935.044
CAGTCAC2600.033.65384727
CGGAGAA2550.032.94117736
CCGGAGA2600.032.30769335
CCAGTCA2750.031.81818226
CTCCAGT2750.030.54545624
Produced by FastQC (version 0.11.5)