FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP25..ph-bZIP9_Col..h-B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP25..ph-bZIP9_Col..h-B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298471
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC23950.10419970493428023TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT8500.027.58790246
TATGCCG8250.027.15118845
CGTATGC8950.025.02762843
TGCCGTC7250.024.6203947
GTATGCC9450.024.44414944
TCGTATG10550.022.22721942
CTCGTAT10400.020.52859741
GCCGTCT6700.020.37288748
GATCTCG10500.018.99977138
TTACTCG13650.018.71772232
CGATCTC11250.018.04422837
GCGTGAA4200.016.66646658
CAGTCAC16050.016.35494224
CTGCGTG2803.916284E-916.24980456
TCTCGTA12750.016.1958840
ATCTCGT13100.016.0303439
CCAGTCA16750.015.46249923
TACTCGA16050.015.26461333
CCGTCTT9350.014.59875449
GCCGTGT2454.2747706E-614.28554148
Produced by FastQC (version 0.11.5)