FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP2..ph..bZIP25C_Col..h_B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP2..ph..bZIP25C_Col..h_B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054012
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT32340.3068276262509345TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGTT23160.21973184366022397TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTTT16970.16100385953860108TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGCGGTT15120.1434518772082291TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT13950.1323514343290209TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTTT13810.13102317620672252TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGGTT13310.1262793971985139TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGTCGTAT12510.11868935078538005TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGCGTTT12440.11802522172423084TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGGGGTT11770.11166855785323127TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGTCGTTT10710.10161174635582897TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAGT53000.062.99974424
CAGTCAC53300.062.90781427
TCCAGTC53400.062.8555525
TGCGGGG5850.062.8202651
ACTCCAG53950.062.27963623
CATTCAG53850.062.26529733
CCAGTCA53700.062.2436926
ACGTCTG54600.062.24333615
CGTCTGA54750.061.94495416
GTCACAT54250.061.6771729
CACGTCT55500.061.42317214
GCGGGGT10150.061.37906352
GAACTCC55000.061.2815721
GCACACG55700.061.2654611
AGTCACA54900.061.13818728
CTGAACT55850.060.59957519
CACATTC56050.060.44578631
CACACGT57000.059.9909812
ACACGTC57200.059.8424113
TCTGAAC56550.059.7875618
Produced by FastQC (version 0.11.5)