FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP2..ph-bZIP9_Col..h-B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP2..ph-bZIP9_Col..h-B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8864894
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT622940.7027043978190828TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG79650.057.4316948
GTATGCC84850.055.5619647
CTCCAGT121850.054.57512724
ACTCCAG122850.054.15967623
GAACTCC124350.053.45128321
CTGAACT124400.053.37322219
ATGCCGT79650.052.94964649
TCACCTG124750.052.91306330
TCGTATG90900.052.8265245
CGTATGC89350.052.8028246
CACCTGA125800.052.443631
CCTGAAG126300.052.40225233
GCTATCT102300.052.20843539
CTCGTAT91700.051.98398644
CTGAAGC128150.051.7550134
AGCTATC111100.051.19188338
TCCAGTC130800.050.97461325
TATCTCG103150.050.48884641
GAGCACA132550.050.4594769
ACCTGAA132100.050.02198432
Produced by FastQC (version 0.11.5)