FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP2..ph-bZIP9_Col..h-B_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP2..ph-bZIP9_Col..h-B_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5910355
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT454080.7682787243744241TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58950.058.12470648
GTATGCC61900.056.82472247
GCTATCT70200.054.74281739
CTCCAGT87650.054.70590624
TCGTATG64550.054.60031545
CGTATGC64650.054.46172346
CTCGTAT64700.053.87867744
CCTGAAG88250.053.77827533
ACTCCAG89050.053.7279423
CACCTGA89100.053.14739631
ATGCCGT60250.053.03659449
CTGAACT90700.052.9816119
GAACTCC90600.052.96327621
CTGAAGC89550.052.8412434
TCACCTG89750.052.68449430
AGCTATC78650.052.3768838
TCCAGTC92950.051.51127225
ACCTGAA92950.051.02133632
TATCTCG73950.050.9729141
GAGCACA97100.049.886019
Produced by FastQC (version 0.11.5)