FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP2..ph-bZIP63_Col..h-B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP2..ph-bZIP63_Col..h-B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527210
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT264701.7332259479704821TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTTT20410.1336423936459295TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31300.064.8547448
CGTATGC33450.062.4649446
GTATGCC34250.061.61902647
ATGCCGT31150.061.57162549
CACCTGA46800.061.4749631
TCACCTG46750.061.46584330
TGCCGTC26400.061.38117250
ACTCCAG47950.060.73052223
CCTGAAG47400.060.54911433
TCGTATG35500.059.64651545
CTCCAGT48750.059.5185324
CTCGTAT34050.059.51405744
AGCTATC41750.059.26810538
CTGAAGC48350.059.21464534
GCTATCT38900.059.02178639
GAACTCC49850.058.90729521
TCCAGTC49650.058.65112725
CTGAACT50050.058.6019719
GTCACCT49950.058.1587329
ACCTGAA49650.058.1576732
Produced by FastQC (version 0.11.5)