FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP2..ph-bZIP63_Col..h-B_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP2..ph-bZIP63_Col..h-B_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364142
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT95242.6154631984225936TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTTT7430.2040412805993266TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATGTCGTAT5180.1422521983182385TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTAT4810.13209132700979287TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11900.066.7639248
CGTATGC12400.066.32986546
GTATGCC12500.066.0792247
CTCCAGT17200.064.30157524
TCACCTG17000.063.82277730
ACTCCAG17450.063.3803423
TCCAGTC17350.063.34219725
TCGTATG13050.063.2942745
GCCGTCT6650.063.1571551
AGCTATC14800.063.14114438
CACCTGA17250.063.10070831
TGCCGTC9900.062.92855550
ATGCCGT11650.062.7889649
GCTATCT13900.062.697139
CTGAACT17650.062.66214819
CTGAAGC17550.062.62034634
CTCGTAT12950.062.43169444
ACGTCTG17850.062.35220715
TCTCGTA12800.062.34301443
CCAGTCA17500.062.19926526
Produced by FastQC (version 0.11.5)