FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP2..ph-bZIP10_Col..h-B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP2..ph-bZIP10_Col..h-B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20072064
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1205740.6007055378061767TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG157700.058.2364248
GTATGCC169150.055.70133247
ATGCCGT158450.054.11746249
CGTATGC174100.053.9769146
GCTATCT201050.052.6428239
CCTGAAG253300.051.55296733
CACCTGA252500.051.50838531
CTCGTAT185000.051.00475744
TGCCGTC142250.051.00475350
TCACCTG255800.050.72087530
ACTCCAG259050.050.57180423
CTCCAGT258600.050.49739524
TATCTCG205550.050.38356841
TCGTATG189950.050.35722445
AGCTATC224050.050.2693938
CTGAAGC261100.050.026334
GAACTCC263800.049.674621
CTGAACT267750.049.0982719
GCCGTCT107450.048.5010151
ACCTGAA273600.047.65119632
Produced by FastQC (version 0.11.5)