FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP2..ph-bZIP10_Col..h-B_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP2..ph-bZIP10_Col..h-B_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13709666
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT789020.5755209499633324TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG104600.056.1465548
GTATGCC112750.053.4849447
ATGCCGT104550.052.49080749
CGTATGC116400.051.35676646
GCTATCT128650.051.20012739
CACCTGA166350.049.7377231
TCACCTG166500.049.52474630
CCTGAAG166950.049.4122233
CTCGTAT121500.049.40269544
CTCCAGT168450.049.22154624
TCGTATG124600.049.07243745
TATCTCG132700.048.87263541
ACTCCAG171850.048.30898723
CTGAAGC171200.048.22646334
AGCTATC145450.048.12574838
GAACTCC173400.048.0591721
TGCCGTC94200.047.8920350
CTGAACT177800.046.9294219
GCCGTCT68200.046.70014651
ACCTGAA181500.045.60534332
Produced by FastQC (version 0.11.5)