FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP11..ph-bZIP10_Col..s-B..h-B_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP11..ph-bZIP10_Col..s-B..h-B_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221038
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2680.12124612057655244TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG800.056.87371448
GCAGTGC200.006945268352.49881418
CGTATGC950.044.2095346
ATGCCGT1050.043.3323549
GTATGCC1000.041.99904647
GTCACGA1300.040.383729
TGCCGTC1050.039.99909250
CTCCAGT1150.039.56432324
ACTCCAG1150.039.56432323
GAACTCC1250.039.19911221
TCACGAG1450.038.61981630
CTGAACT1400.037.4991519
CTCGTAT951.8553692E-1036.84127444
TCGTATG1151.8189894E-1236.52091245
TCCAGTC1250.036.39917825
AGCACAC1500.034.9992110
TCTGAAC1500.034.9992118
CGTCTGA1500.034.9992116
GCACACG1500.034.9992111
ACGTCTG1500.034.9992115
Produced by FastQC (version 0.11.5)