FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP10..ph-vec_Col..h_B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP10..ph-vec_Col..h_B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248260
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG8640.34802223475388705TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG1400.049.9989942
ACTCCGG1550.049.6764232
CGGGGGG1400.044.99909244
CACTCCG1900.044.20963731
TCCGGAG1750.043.99911534
CAGTCAC1850.041.62078527
GAATCGG1700.041.1756440
AATCGGG1700.041.1756441
TCGGGGG1750.039.99919543
CTCCGGA1950.039.48638533
CCAGTCA1900.038.6834326
AGTCACT2000.036.7492628
TCCAGTC2050.035.85293625
ACTCCAG2000.034.99929423
CCGGAGA2350.034.2546335
GTCACTC2300.033.4775929
CTCCAGT2200.031.81753724
GGAGAAT2350.031.27596737
CTCCCTG450.004302443531.11048570
ACCTGTA450.004302443531.11048517
Produced by FastQC (version 0.11.5)