FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP10..ph-bZIP9_Col..h-B_q_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP10..ph-bZIP9_Col..h-B_q_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603790
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3650.025.89054948
CGTATGC4600.022.82620846
TGCCGTC3550.022.67688450
GCCGTCT3100.021.45239451
GTATGCC4850.020.92794647
ATGCCGT4400.020.68257349
TCGTATG5750.016.43435942
CTCGTAT6250.013.99963944
GATCTCG6850.013.79526441
CAGTCAC8750.013.59964827
TTACTCG9350.012.35262235
CCAGTCA9800.012.14254326
CCGTCTT5451.382432E-1011.56005552
TCCAGTC10050.011.49224125
ATCTCGT8350.011.31707242
CTCGATC9300.011.290030538
GTCACAT11000.011.13607729
CGATCTC7900.011.07566440
CTCCAGT11000.010.81790324
ACTCCAG10450.010.71742623
Produced by FastQC (version 0.11.5)