FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_ps-bZIP10..ph-bZIP11_Col..s-B..h-B_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_ps-bZIP10..ph-bZIP11_Col..s-B..h-B_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7270150
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGC5200.013.46127763
AGGGGGG4905.251495E-710.0013881
CCCGCGT7604.303365E-88.28931265
CGGGCAG8151.6190825E-88.1593556
GCCCGCG9252.2591848E-97.945791264
AGCGCGC4450.00180485657.86501557
AACGGGC9352.7848728E-97.8608154
GCCCTAT11152.3646862E-117.84738145
GAGCCCG9604.649337E-97.656100762
CCCGATC46000.07.532462658
CAAGGGG9553.4082404E-87.329731
CAACCCG46850.07.246390355
GCGGGCG18550.07.16972167
ACGCCCG6351.456619E-47.16521569
AGGAGCG19200.07.109236268
AGCCTAA95400.07.00720170
GCAACCC48300.06.88392454
AAGCCTA96950.06.786871469
ACCCGAT46600.06.759525357
GACGCCC7251.0043958E-46.75848968
Produced by FastQC (version 0.11.5)