FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_pIXHALO_PN94-B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_pIXHALO_PN94-B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6612998
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6050.013.30556750
GCCGTCT4853.1941454E-911.54620251
TATGCCG7650.010.98021348
ATGCCGT8505.456968E-129.47043349
CGTATGC9400.09.30835846
TATCTCG14200.07.887194641
CGCTCAT15100.07.88066534
CCGCTCA14900.07.751550733
ACCGCTC14550.07.697468832
TCACCGC17550.06.780515730
CACCGCT15703.6379788E-126.687788531
CTCGTAT13102.7083843E-75.87776644
TCTCGTA16851.1095835E-95.81592843
CCGTCTT10304.3651293E-55.77660452
GTATGCC17352.2591848E-95.64832247
TCGTATG15006.4493724E-75.366578645
GTCACCG19651.4842954E-95.34342429
ACACGTC60150.05.178634670
ATCTCGT19553.9390216E-85.01270542