FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_pIXHALO_PN94-B_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_pIXHALO_PN94-B_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8399801
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9000.013.61090945
TGCCGTC7200.013.124804547
ATGCCGT9900.013.08061346
GCCGTCT6603.274181E-1110.60590348
CGTATGC10900.09.9539843
TATCTCG18550.07.924457638
CCGCTCA21350.07.70480430
CTCGTAT17650.07.53534341
CGCTCAT21850.07.52849231
TCGTATG18450.07.01890842
TCTCGTA19500.06.999937540
ACCGCTC21600.06.967489229
GTATGCC20450.06.50357144
CCGTCTT13055.7461875E-96.43668649
ATCTCGT24000.06.41660939
GTCACCG26150.06.290537426
CACCGCT23400.06.281957628
GATTCGC7903.0641418E-46.202439335
TCACCGC25500.05.901872627
TGCCGTG7200.00464835955.83324647
Produced by FastQC (version 0.11.5)