FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_pIXHALO_Colamp-B_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_pIXHALO_Colamp-B_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171498
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATC5000.048.999649
GTGGCCA5800.047.6720667
GGTGGCC2850.042.9858136
TGGCCAT6200.042.3383988
GCCATCT6200.039.5158410
CCGTCCA2950.039.1522567
AGTGGCC3000.029.1689436
CGTCCAT4000.027.9997948
GTGCCCC500.00721068227.99979438
CCATCTG4150.025.3010211
GGCCGTC1251.0994154E-725.2019675
GTCCATC4200.023.3331629
GTATGCC5250.021.99983868
CCATCTA6800.021.61748711
TATGCCG5200.021.53830369
TGTGGCC2151.1459633E-1021.1644426
CCCCCCA850.00379096920.58808365
AGGTGGC2402.3646862E-1120.4182625
CGTATGC5550.020.1800367
ATGCCGT4900.019.99985370
Produced by FastQC (version 0.11.5)