FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_pIXHALO_Col-B_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_pIXHALO_Col-B_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212140
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT12670.5972471009710568TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT150.00222239770.000013
TGCCGTC1100.063.63636850
GCCGTCT750.060.66666851
ATGCCGT1000.059.50000449
TATGCCG1100.057.2727348
CGCTCAT1800.056.38889334
CCGCTCA1900.053.4210533
AACGCGA200.006944263452.52
TCGTATG1400.052.545
ACCGCTC1950.052.0512832
GTCACCG2000.050.75000429
CTCGTAT1450.050.68965544
CGTATGC1350.049.2592646
CACCGCT2100.048.33333631
CCAGTCA2100.048.33333626
AGTCACC2150.047.209328
GCTCATT2150.047.209335
ACTCCAG2200.046.13636823
CCGTCTT1000.045.50000452
ACGTCTG2200.044.54545615
Produced by FastQC (version 0.11.5)