FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_pIXHALOC_Col-B_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_pIXHALOC_Col-B_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275497
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT33621.2203399674043638TruSeq Adapter, Index 7 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC2770.10054555947977654TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5300.062.7358548
ATGCCGT5000.060.90000549
CGTATGC5650.060.08849746
GTATGCC5600.060.047
CTCGTAT5400.058.9814844
TCGTATG5850.058.03418745
GTCACGA6850.057.7372329
TGCCGTG1700.057.6470650
TGGGGGT553.6379788E-1157.27272458
AGTCACG7050.057.09219728
TCCAGTC7200.056.38889325
CCAGTCA7200.056.38889326
TCACGAG6900.056.30434430
TCTCGTA5350.056.26168443
TGCCGTC4000.055.12499650
TGCCGGC452.6935595E-854.44444750
CAGTCAC7400.054.3918927
ATCTCGT5800.054.31034542
GCCGTCT2650.054.15094451
CTCCAGT7650.053.9869324
Produced by FastQC (version 0.11.5)