FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_daplib_poolR7_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_daplib_poolR7_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6732314
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAATC1582250.068.8269659
GACCAAT1237050.068.434238
CCAATCT1246800.068.3683710
TGTCAAT1594850.068.31788
ACTTGAA1955550.068.277587
CAATCTA1294300.068.142111
ATGTCAA1601900.068.113787
ACCAATC1242000.068.074639
TGACCAA1245850.068.041257
CCGTCCA246850.068.000167
TCCATCT1137500.067.85474410
CTTGAAT1968550.067.8279658
TGAATCT1964650.067.8063410
AGTTCCA899850.067.770657
TCAATCT1607500.067.7393210
TTGAATC1970400.067.667089
CCATCTA578200.067.63302611
GTTTCGT802150.067.625737
GTTCCAT901850.067.5499958
GTGACCA366350.067.445486
Produced by FastQC (version 0.11.5)