FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_daplib_poolR6_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_daplib_poolR6_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550884
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAATC837350.069.192449
GACCAAT657900.068.754298
ATGTCAA845750.068.6997157
TGTCAAT844350.068.685138
CAATCTA682350.068.68091611
CCAATCT662000.068.6615410
ACTTGAA1033350.068.640957
TGACCAA659850.068.625367
GTGACCA198000.068.469336
TCAATCT845750.068.4638310
ACCAATC660100.068.456219
TGAATCT1037900.068.2388810
CTTGAAT1040550.068.1693658
TTGAATC1040250.068.094819
CAATCTG190000.067.49390411
CAATCTC341350.067.4870811
GAATCTA444200.067.2492811
GGTGACC60250.066.9863055
CTGACCA129900.066.827676
AATCTAG222750.066.7793912