FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_daplib_poolR5_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_daplib_poolR5_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3851745
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAATC1251300.069.137379
GACCAAT997250.068.77058
ACTTGAA1574250.068.716047
CCAATCT1005900.068.71236410
TGACCAA1001000.068.57937
CAATCTA1034050.068.5680211
TGTCAAT1262700.068.549218
ACCAATC1000450.068.5015649
TGAATCT1576700.068.4592110
ATGTCAA1266900.068.421417
CTTGAAT1582950.068.3361748
TTGAATC1580150.068.326589
GTGACCA298400.068.2565466
TCAATCT1267150.068.2403110
GAATCTA681300.067.55958611
CAATCTC506550.067.21525611
CAATCTG292350.067.1743911
TATGTCA408300.066.7209856
CATGTCA287750.066.720016
CTGACCA202700.066.688866
Produced by FastQC (version 0.11.5)