FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_daplib_poolR4_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_daplib_poolR4_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876905
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAATC1936400.069.077179
ACTTGAA2385550.068.813537
GACCAAT1502500.068.783038
CCAATCT1513600.068.7746110
TGTCAAT1951100.068.633878
ACCAATC1503400.068.597519
CAATCTA1571150.068.579511
ATGTCAA1956400.068.5427557
TGACCAA1509100.068.519317
TGAATCT2394400.068.466810
CTTGAAT2401400.068.369538
TCAATCT1959200.068.27624510
TTGAATC2401300.068.265989
GTGACCA445100.068.095156
GAATCTA1040150.067.70251511
CAATCTC788850.067.334211
CAATCTG446300.067.04420511
CTGACCA306550.066.877486
CACTTGA504800.066.858066
ATGACCA394750.066.654346
Produced by FastQC (version 0.11.5)