FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_daplib_poolR3_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_daplib_poolR3_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143660
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGAA1151400.069.044557
CTTGAAT1155900.068.775748
AGTTCCA517500.068.6869667
GTTTCGT448050.068.671067
TTGAATC1156150.068.667039
TGAATCT1154850.068.6534110
GTTCCAT518700.068.447088
TTCCATC517650.068.356049
TCCATCT516200.068.2768510
GAATCTA486350.068.15674611
CCATCTA257900.068.0040911
CACTTGA240600.067.997916
GAGTTCC145400.067.838946
CGTTTCG91700.067.7152946
GGTTTCG140900.067.5461656
TAGTTCC149650.067.5028466
TACTTGA354600.067.3206256
TTTCGTA233200.067.222478
CAGTTCC115550.067.1277856
CGACTTG61100.066.912025
Produced by FastQC (version 0.11.5)