FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_daplib_poolR2_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_daplib_poolR2_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3791361
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGAA2022950.068.968967
CTTGAAT2027950.068.8075568
AGTTCCA926200.068.732487
TGAATCT2026500.068.65816510
TTGAATC2030550.068.605689
GTTCCAT926750.068.582188
GTTTCGT809150.068.50617
TCCATCT925800.068.26694510
TTCCATC929450.068.266219
GAATCTA872950.068.0788211
CCATCTA455950.067.94208511
CACTTGA422550.067.812366
GAGTTCC260100.067.6794056
CGTTTCG164000.067.4890446
TAGTTCC269150.067.4195566
TACTTGA594900.067.383366
GAATCTC441300.067.1599911
CGACTTG114050.066.999835
CAGTTCC203300.066.994496
GGTTTCG258200.066.957316
Produced by FastQC (version 0.11.5)