FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_daplib_poolR1_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_daplib_poolR1_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5294228
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGAA2826200.068.934817
CTTGAAT2831400.068.82058
TGAATCT2829350.068.6928910
AGTTCCA1290050.068.610757
GTTCCAT1288050.068.577418
TTGAATC2837050.068.574889
GTTTCGT1141750.068.528427
TTCCATC1288400.068.3659069
TCCATCT1284900.068.2531510
GAATCTA1217000.068.1276211
CCATCTA644350.068.0498211
CACTTGA594350.067.9241946
GAGTTCC358150.067.800246
TACTTGA834100.067.377786
CGTTTCG227900.067.367246
TAGTTCC380600.067.213156
GGTTTCG364500.067.147276
TTTCGTA585150.067.019868
GAATCTC626750.066.9842911
GCACTTG189650.066.834415
Produced by FastQC (version 0.11.5)