FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_daplib_Col-input_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_daplib_Col-input_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878825
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATC4100.047.8068589
GTGGCCA4500.045.1104167
TGGCCAT5000.039.8993848
GGTGGCC2050.039.2721566
GCCATCT5200.037.02076710
CCGTCCA2250.034.2216957
CCATCTA4800.027.70948411
GTATGCC4250.023.88198568
TATGCCG4300.022.79034669
CGTGGCC1854.0381565E-1022.7049356
CCCGTCC1102.9202296E-522.274926
CGTATGC4250.022.23495167
AGTGGCC2259.094947E-1221.779926
GCCGTCT2259.094947E-1221.77744368
ATGCCGT4100.021.34113370
TGTGGCC1656.7882866E-821.2142076
CCATCTG3000.021.0008711
GTCCATC2950.020.1703269
CGTCCAT3350.019.8504418
ACCGTCC1501.494551E-518.6685036
Produced by FastQC (version 0.11.5)