FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_bZIP63_Col-B_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_bZIP63_Col-B_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422461
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT78901.8676280177341815TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT5410.12805915812347174TruSeq Adapter, Index 7 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC5220.12356170155351617TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTTT4290.10154783518478629TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11250.059.73000348
CGTATGC11700.058.92833346
ATGCCGT10900.058.4371149
TGCCGTC9450.058.1449150
GTATGCC11950.057.98839647
TCACGAG15200.057.10883330
GTCACGA15100.057.01668529
AGTCACG15150.056.597528
GCCGTCT6250.056.55684351
CACGAGA15300.056.27803431
TCGTATG12200.055.93950345
CATCTCG11150.055.87132341
CTCGTAT11350.055.81186344
CAGTCAC15400.055.6787127
CCAGTCA15500.055.32604626
ACGAGAT15500.055.32604632
GCGTGAA2600.055.18922861
CGAGATT15450.055.05198733
TCGCGTA700.054.9969343
ACTCCAG15600.054.74701723
Produced by FastQC (version 0.11.5)