FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_bZIP25C_Col-B_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_bZIP25C_Col-B_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176321
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT27201.5426409786695856TruSeq Adapter, Index 7 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC2070.11739951565610449TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT2010.11399663114433334TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT207.910043E-570.051
GCCGTCT2700.060.92592651
GTATGCC4100.060.60975647
TATGCCG4050.060.49382848
ATGCCGT3950.060.25316649
TGCCGTC3300.059.3939450
GTCACGA5150.059.12621729
AGTCACG5350.057.5700928
TCACGAG5300.057.4528330
CACGAGA5300.057.4528331
CGTATGC4400.057.27272446
CCGTCTT3000.057.16666852
CAGTCAC5450.057.15596427
CTCGTAT4350.057.1264444
GCGTGAA750.056.061
GGGTGTA252.3854696E-455.99999661
TTGTGCG252.3854696E-455.99999657
CCAGTCA5600.055.62500426
TCCAGTC5650.055.13274425
ACTCCAG5650.055.13274423
Produced by FastQC (version 0.11.5)